AMMONIUM-MEDIATED REDUCTION OF PLASMID COPY NUMBER AND RECOMBINANT GENE-EXPRESSION IN ESCHERICHIA-COLI

Authors
VILA P CORCHERO JL BENITO A VILLAVERDE A
Citation
P. Vila et al., AMMONIUM-MEDIATED REDUCTION OF PLASMID COPY NUMBER AND RECOMBINANT GENE-EXPRESSION IN ESCHERICHIA-COLI, Biotechnology progress, 10(6), 1994, pp. 648-651
Citations number
15
Categorie Soggetti
Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Biotechnology progress → ACNP
ISSN journal
87567938
Volume
10
Issue
6
Year of publication
1994
Pages
648 - 651
Database
ISI
SICI code
8756-7938(1994)10:6<648:AROPCN>2.0.ZU;2-I
Abstract
The effect of ammonium as a medium supplement on plasmid-encoded recom binant beta-galactosidase synthesis was explored in Escherichia coli c ells during aerobic growth in complex medium. After induction, only do ses of ammonium chloride below 1 g/L are able to transiently enhance t he yield. However, the presence of nontoxic ammonium chloride concentr ations of up to 10 g/L results in lower values of beta-galactosidase i n a concentration-dependent fashion. A significant reduction in plasmi d DNA content explains the decrease in the yield by a gene-dosage-invo lving mechanism.