J. Zavadova et S. Svrcek, THE IMPROVEMENT OF LABORATORY DIAGNOSTICS OF RABIES AND TITRATION OF RABIES VIRUS, Veterinarni medicina, 39(11), 1994, pp. 663-676
For primary isolation and titration of street strains of the rabies vi
rus from brains of suspected animals, an assay prepared on the cell cu
lture BHK-21/C 13 (rabies infection test - RTCIT) was used. The above
assay proved to be reliable and its sensitivity proved to be comparabl
e to the standard mouse inoculation test. Through this test, the resul
ts were obtained within 24 to 48 hrs on Lab-Tek tissue culture chamber
/slides. It was found out that DEAE-dextran added to the cell culture
only slightly increased the invasiveness of the virus in the samples t
ested. The method described herein is able to substitute the mouse ino
culation test (MIT). In our laboratory, 20 vaccination strains of the
rabies virus Vnukovo-32/107 and 25 street strains of the rabies virus
(delivered from the field) - original fox brain suspensions. And 10 br
ain suspensions were negative when tested in laboratory conditions (by
PMIF, RTCIT as well as by MIT methods).