CHANGES IN GLOMERULAR EPITHELIAL-CELLS INDUCED BY FGF2 AND FGF2 NEUTRALIZING ANTIBODY IN PUROMYCIN AMINONUCLEOSIDE NEPHROPATHY

In the present study, two series of experiments were done with PAN nep hropathy rats given fibroblast growth factor 2 (FGF?) or FGF2 neutrali zing antibodies. Ln the first series of experiments, a dose of 10 mu g of FGF2 (FGF2 group), 40 mu g of an FGF2 neutralizing antibody (Anti- FGF2 group) or an equal volume of physiological saline (Control group) was administered for four days after PAN injection. Urinary protein i ncreased more in the FGF2 group than in the other two groups. PCNA (+) glomerular cells were found in decreasing order in groups FGF2, Contr ol and Anti-FGF2. Most of the PCNA (+) cells were podocytes and epithe lial cells of Bowman's capsule. Staining for desmin, a marker of podoc yte injury, was significantly reduced in the Anti-FGF2 group. Glomerul ar adhesive lesions were found in decreasing order in groups FGF2, Con trol and Anti-FGF2. The second series of experiments was designed to s tudy the effects of FGF2 neutralizing antibody (40 mu g for 5 days aft er PAN injection, in MoAb group) on severely damaged podocytes caused by repeated (two courses) injections in the PAN nephropathy rats. The results were the same as those in series 1. An increase in urinary pro tein excretion was observed in both groups, but on the 40th day, the l evel of proteinuria in the MoAb group decreased abruptly. It was obser ved that the MoAb group had few adhesive glomeruli compared to the IgG group (administration of mouse IgG) and the PCNA (+) epithelial cells of Bowman's capsule were also few. It was supposed that FGF2 would pr omote the formation of adhesive lesions by stimulating the proliferati on of podocytes and epithelial cells of Bowman's capsule. Additionally , FGF2 itself was thought to impair podocytes because of the increasin g desmin score and proteinuria.