Wy. Xuvanopstal et al., AUTOMATIC CELL-CULTURE QUANTITATION WITH TRAKCELL - APPLICATION TO CELL TOXICOLOGY AND DIFFERENTIATION, Cell biology and toxicology, 10(5-6), 1994, pp. 387-392
An automated system, TRAKCELL, was developed for the quantitation of c
ells in culture. It enabled cell counting, classification according to
morphological cell characteristics and measurement of cell proliferat
ion and differentiation. The system was tested on the toxic effect of
ascorbic acid on rat brain catecholaminergic neurons in primary cultur
e. In parallel, the effects of nerve growth factor, dexamethasone and
forskolin on cell differentiation were studied using rat pheochromocyt
oma PC12 cells. The results show that the system permits rapid and rep
roducible measurements of cell density and of the morphological change
s observed following various drug treatments.