MOLECULAR-CHANGES OF ORGANELLE DNA-SEQUENCES IN RICE THROUGH DEDIFFERENTIATION, LONG-TERM CULTURE, OR THE MORPHOGENESIS PROCESS

Callus-specific rearranged DNA in rice (Oryza sativa L.) was isolated by in-gel reassociation procedure. Southern hybridization experiments revealed that some clones were amplified significantly in primary call us induced from scutellum tissue. Rapid amplification of these clones was observed within 2 days after plating seeds onto callus-induction m edium containing 2,4-D. NAA gave no significant effect on BNA amplific ation event. Colony formation process from isolated protoplasts and pl ant regeneration process from callus showed clone-specific and process -specific fluctuation patterns of copy number. Sequence analysis of th e clones suggested that most of the clones were originated from organe lle DNA. comparison of copy number fluctuation pattern of organelle fu nctional genes with that of the clones suggested multiformity and/or c onstruction-specific amplification of organelle DNA.