S. Krishnasamy et Ca. Makaroff, ORGAN-SPECIFIC REDUCTION IN THE ABUNDANCE OF A MITOCHONDRIAL PROTEIN ACCOMPANIES FERTILITY RESTORATION IN CYTOPLASMIC MALE-STERILE RADISH, Plant molecular biology, 26(3), 1994, pp. 935-946
The mitochondrial DNA of plants containing the male sterility-causing
Ogura cytoplasm of radish contain a novel gene, orf138, that is transc
ribed as part of a bicistronic mRNA. Genetic studies have previously l
inked male sterility with the orf138 locus. To determine if orf138 is
expressed at the protein level, and investigate the effect of fertilit
y restoration on ORF138 levels, we have raised antibodies to an ORF138
-glutathione S-transferase fusion protein. Anti-ORF138 antibodies dete
ct a 20 kDa protein that is associated with the mitochondrial membrane
of sterile Ogura radish plants. Nuclear restoration is accompanied by
a dramatic reduction in the amount of this protein in mitochondria of
flowers and leaves, but not roots of fertile Ogura radish plants. The
presence or absence of fertility restoration genes has no detectable
effect on the size, abundance, or RNA editing patterns of orf138 trans
cripts. These results support genetic studies that have implicated orf
138 in Ogura cytoplasmic male sterility and suggest that the restorer
genes may be affecting either the translation or stability of ORF138.