Ag. Hepburn, SIZE - A PROGRAM TO DETERMINE THE SIZE OF NUCLEIC-ACID AND PROTEIN MOLECULES FROM GEL MOBILITIES, BioTechniques, 17(6), 1994, pp. 1140-1142
The relationship between fragment length and the reciprocal of mobilit
y and the least-squares curve fitting analysis for this relationship h
ave been combined into a user-friendly program for determining the siz
e of unknown DNA fragments by reference to known standards. Protein si
zes can be determined in the same way. The program offers an accurate,
simple-to-use mechanism for sizing molecules that requires only an IB
M(R)-compatible computer.