SIMULTANEOUS DETERMINATION OF POLYCYCLIC AROMATIC-HYDROCARBONS AND THEIR NITRATED DERIVATIVES IN AIRBORNE-PARTICULATES BY HPLC WITH FLUORESCENCE CHEMILUMINESCENCE DETECTION/

A simultaneous determination method for polycyclic aromatic hydrocarbo ns (PAHs) and nitro-PAHs (NPAHs) in airborne particulates was develope d. Airborne particulates were extracted with benzene/ethanol, cleaned up by liquid-liquid partition, refluxed in the presence of NaSH and th en the resultant solution was injected into an HPLC system. The system was hybridized into two systems with different optimum conditions for PAHs and NPAHs by introducing a switching valve and a mini separation column. Mobile phases were acetonitrile/water (75 : 25) for PAHs, and acetonitrile/imidazole HClO4 buffer (1 : 1) for NPAHs and the postcol umn reagent solution for NPAHs was an acetonitrile solution containing bis(2,4,6-trichlorophenyl)oxalate and H2O2 By this method, four NPAHs (1,3-, 1,6-, 1,8-dinitropyrenes and 1-nitropyrene) and eight PAHs (fl uoranthene, pyrene, benz[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k] fluoranthene, benzo[a]pyrene and coronene) in sub-milligram quantities of airborne particulates were determined chemilumigenically and fluorogenically, respectively using 2-fluoro-7-nitrofluorene as a n internal standard within sixty minutes.