ALTERATIONS OF YEAST ARTIFICIAL CHROMOSOME TRANSGENIC SEQUENCES IN STRETCHED EMBRYONIC STEM-CELL CHROMATIN VISUALIZED BY FLUORESCENCE IN-SITU HYBRIDIZATION

Authors
ROSENBERG C VOLTZ AK LAWLER AM LAMB BT STETTEN G GEARHART JD
Citation
C. Rosenberg et al., ALTERATIONS OF YEAST ARTIFICIAL CHROMOSOME TRANSGENIC SEQUENCES IN STRETCHED EMBRYONIC STEM-CELL CHROMATIN VISUALIZED BY FLUORESCENCE IN-SITU HYBRIDIZATION, Cytogenetics and cell genetics, 75(1), 1996, pp. 67-70
Citations number
14
Categorie Soggetti
Cell Biology","Genetics & Heredity
Journal title
Cytogenetics and cell genetics → ACNP
ISSN journal
03010171
Volume
75
Issue
1
Year of publication
1996
Pages
67 - 70
Database
ISI
SICI code
0301-0171(1996)75:1<67:AOYACT>2.0.ZU;2-2
Abstract
Transgenic mice have been generated from embryonic stem (ES) cells car rying functional genes cloned within yeast artificial chromosomes (YAC s). Information on the integrity and organization of the inserted sequ ences, including the number of copies and their orientation to each ot her, is still limited by current methods. We have applied fluorescence in situ hybridization to stretched chromatin preparations from YAC-tr ansfected ES cells to analyze the organization and copy number of the integrated sequences.