MOLECULAR KARYOTYPE ANALYSIS OF CRYPTOSPORIDIUM-PARVUM - EVIDENCE FOR8 CHROMOSOMES AND A LOW-MOLECULAR-SIZE MOLECULE

Authors
BLUNT DS KHRAMTSOV NV UPTON SJ MONTELONE BA
Citation
Ds. Blunt et al., MOLECULAR KARYOTYPE ANALYSIS OF CRYPTOSPORIDIUM-PARVUM - EVIDENCE FOR8 CHROMOSOMES AND A LOW-MOLECULAR-SIZE MOLECULE, Clinical and diagnostic laboratory immunology, 4(1), 1997, pp. 11-13
Citations number
16
Categorie Soggetti
Immunology,"Infectious Diseases","Medical Laboratory Technology",Microbiology
Journal title
Clinical and diagnostic laboratory immunology → ACNP
ISSN journal
1071412X
Volume
4
Issue
1
Year of publication
1997
Pages
11 - 13
Database
ISI
SICI code
1071-412X(1997)4:1<11:MKAOC->2.0.ZU;2-7
Abstract
We report improved separation of chromosome-sized DNA molecules of the coccidian parasite Cryptosporidium parvum with contour-clamped homoge neous electric fields (CHEF). We used scanning densitometry to determi ne that the most likely number of chromosomes is eight. Molecular prob es consisting of cloned genes were used to distinguish each of five ba nds visible on CHEF gels. We have also identified a low-molecular-size DNA molecule possibly related to the 35-kb circular DNAs found in oth er Apicomplexa.