AMPLIFICATION ON CHROMOSOMES 1P31, 1Q21-24, 5P13-14, AND 11P12-14 IN OVARIAN-CARCINOMA DETECTED BY REVERSE CHROMOSOME PAINTING

Authors
FISCHER U SATTLER HP BONKHOFF H HERRMANN M WULLICH B MEESE EU
Citation
U. Fischer et al., AMPLIFICATION ON CHROMOSOMES 1P31, 1Q21-24, 5P13-14, AND 11P12-14 IN OVARIAN-CARCINOMA DETECTED BY REVERSE CHROMOSOME PAINTING, Oncology Reports, 1(6), 1994, pp. 1069-1073
Citations number
36
Categorie Soggetti
Oncology
Journal title
Oncology Reports → ACNP
ISSN journal
1021335X
Volume
1
Issue
6
Year of publication
1994
Pages
1069 - 1073
Database
ISI
SICI code
1021-335X(1994)1:6<1069:AOC115>2.0.ZU;2-4
Abstract
Recently, amplifications of several genes including c-myc, HER-2/neu, and FGF-3 (int-2) have been identified in ovarian carcinomas. We analy zed Il tumor samples from ovarian carcinoma for gene amplification usi ng reverse chromosome painting and standard Southern blot analysis. Re verse chromosome painting detected four amplified domains on chromosom e bands 1p31, 1q21-24, 5p13-14, and 11p12-14. None of the amplified do mains contained genes previously reported to be amplified in ovarian c arcinomas. Southern blot analysis revealed amplifications of genes HER 2/neu, N-ras and H-ras. Tumor T3711 showed three independent amplifica tions including chromosome bands 1p31, 11p12-14 and the HER-2/neu gene (17q11.2-12).