RETROVIRUS-MEDIATED GENE-TRANSFER OF TNF RECEPTOR FOR TREATMENT OF CANCER

Authors
FAN ZH ISOBE K EMI N NAKASHIMA I
Citation
Zh. Fan et al., RETROVIRUS-MEDIATED GENE-TRANSFER OF TNF RECEPTOR FOR TREATMENT OF CANCER, Oncology Reports, 1(6), 1994, pp. 1113-1116
Citations number
19
Categorie Soggetti
Oncology
Journal title
Oncology Reports → ACNP
ISSN journal
1021335X
Volume
1
Issue
6
Year of publication
1994
Pages
1113 - 1116
Database
ISI
SICI code
1021-335X(1994)1:6<1113:RGOTRF>2.0.ZU;2-R
Abstract
Tumor necrosis factor-alpha receptor cDNA (TNFR-1 or TNFR-2) was inser ted into a retrovirus derived vector. Recombinant vector was transfect ed into packaging cell line. The transfectants producing high titer of virus containing TNFR-1 or TNFR-2 cDNA were obtained. Human stomach c ancer cells that poorly expressed TNFR-1 or TNFR-2 were infected with this virus as an in vitro model experiment of gene therapy. The stomac h cancer cells that were infected with recombinant virus expressed TNF R-1 or TNFR-2 and were effectively killed by recombinant TNF.