H. Iguchi et al., ALTERATIONS OF THE P53 TUMOR-SUPPRESSOR GENE AND KI-RAS ONCOGENE IN HUMAN PANCREATIC CANCER-DERIVED CELL-LINES WITH DIFFERENT METASTATIC POTENTIAL, Oncology Reports, 1(6), 1994, pp. 1223-1227
Alterations of the p53 and Ki-ras genes were examined in 12 human panc
reatic cancer-derived cell lines with different metastatic potential.
Point mutations of the Ki-ras gene at codon 12 were found in 10 out of
12 cell lines (83%), while abnormalities of the p53 gene were identif
ied in 8 out of 12 cell lines (67%) which included point mutations (n=
7) and one base deletion (n=1). The comparison between alterations of
the p53 and Ki-ras genes showed that all the 12 cell lines revealed al
terations of both genes or one of these genes regardless of the metast
atic potential. Further, same alterations of the Ki-ras gene or p53 ge
ne were noted among the cell lines with increased metastatic potential
and their parental cell lines. These findings suggest that alteration
s of the p53 gene, like the Ki-ras gene is a frequent event in pancrea
tic cancer, and could contribute cooperatively in the oncogenic steps
of pancreatic cancer. It is also suggested that the genetic changes of
the p53 and Ki-ras genes are not substantially associated with the me
tastatic potential in pancreatic cancer.