Jyk. Hsieh et al., ROBOTIC SAMPLE PREPARATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF VERLUKAST IN HUMAN PLASMA, Journal of chromatography B. Biomedical applications, 661(2), 1994, pp. 307-312
Citations number
7
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
A fully automated HPLC assay has been developed and validated for the
quantitation of verlukast, a leukotriene D-4, antagonist, in human pla
sma. An upgraded Zymate I robotic system was utilized to perform prote
in precipitation and on-line injection followed by reversed-phase HPLC
with fluorescence detection. Inter-day accuracy and precision were 10
0.8 and 4.6%, respectively, for the low quality control standards (0.1
25 mu g/l). The automated robotic method was shown to be more efficien
t and accurate than the manual method.