Mj. Navarrolozano et al., KINETIC-STUDY OF AN ENZYME-CATALYZED REACTION IN THE PRESENCE OF NOVEL IRREVERSIBLE-TYPE INHIBITORS THAT REACT WITH THE PRODUCT OF ENZYMATIC CATALYSIS, Bulletin of mathematical biology, 57(1), 1995, pp. 157-168
In the present paper a kinetic study is made of the behaviour of a Mic
haelis-Menten enzyme-catalysed reaction in the presence of irreversibl
e inhibitors rendered unstable in the medium by their reaction with th
e product of enzymatic catalysis. A general mechanism involving compet
itive, non-competitive, uncompetitive and mixed irreversible inhibitio
n with one or two steps has been analysed. The differential equation t
hat describes the kinetics of the reaction is non-linear and computer
simulations of its dynamic behaviour are presented. The results obtain
ed show that the systems studied here present kinetic co-operativity f
or a target enzyme that follows the simple Michaelis-Menten mechanism
in its action on the substrate, except in the case of an uncompetitive
-type inhibitor.