The identification of genes of large effect on ecologically important
traits is an important aim of molecular ecology. The period gene of Dr
osophila is a candidate for a gene with a large influence on premating
isolation between Drosophila species, as it determines species specif
ic aspects of courtship behaviour. Strains of D. melanogaster are avai
lable which have been genetically transformed with the period gene of
either D. melanogaster or D. simulans. Here we show that D. melanogast
er females do not discriminate between two such strains. This suggests
that period may only make a small contribution to total premating iso
lation between these species. We discuss the use of genetically transf
ormed strains in assessing the influence of single genes on complex tr
aits.