SEROLOGICAL ANALYSIS OF PULMONARY AND EXTRAPULMONARY TUBERCULOSIS WITH ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR ANTI-A60 IMMUNOGLOBULINS

Citation
Yl. Zou et al., SEROLOGICAL ANALYSIS OF PULMONARY AND EXTRAPULMONARY TUBERCULOSIS WITH ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR ANTI-A60 IMMUNOGLOBULINS, Clinical infectious diseases, 19(6), 1994, pp. 1084-1091
Citations number
49
Categorie Soggetti
Microbiology,Immunology,"Infectious Diseases
ISSN journal
10584838
Volume
19
Issue
6
Year of publication
1994
Pages
1084 - 1091
Database
ISI
SICI code
1058-4838(1994)19:6<1084:SAOPAE>2.0.ZU;2-N
Abstract
IgA, IgG, and IgM antibodies to mycobacterial antigen A60 were measure d by ELISA in blood, pleural fluid, and cerebrospinal fluid from 560 p atients with pulmonary and/or extrapulmonary tuberculosis who were bei ng treated at hospitals in northern China and from 734 uninfected cont rols. Among 529 healthy persons (most of whom had been vaccinated with bacille Calmette-Guerin [BCG] and 287 of whom were tuberculin-positiv e), the rate of false-positive results was negligible; this observatio n ruled out interference of remote BCG vaccination with A60 assays at the chosen cutoff level. Rates of positivity for IgM and IgG, respecti vely, were 80% and 36% among patients with active primary pulmonary tu berculosis, 31% and 88.5% among patients with active postprimary. pulm onary tuberculosis, 0 and 41% among patients with inactive pulmonary t uberculosis, and 30%-61% and 69%-86% among patients with extrapulmonar y tuberculosis. Paired samples of blood and pleural fluid from patient s with pleurisy contained IgA antibody to A60 at equal titers; in cont rast, most patients with tuberculous meningitis (100% of whom had a po sitive ELISA result) had higher levels of IgG antibody to A60 in cereb rospinal fluid than in blood-proof of intrathecal synthesis.