ANTISENSE GENE-EXPRESSION IN YEAST

The use of antisense and ribozyme RNA to modulate gene expression is e merging as an effective genetic technique. A compilation of successful antisense gene suppression experiments reveals the absence of reports on the use of the yeast Saccharomyces cerevisiae as a host. We examin e the field of antisense and ribozyme use in S. cerevisiae and discuss that this result is not due to any lack of attempts and may reflect u nique features of S. cerevisiae biology. in an attempt to learn from c ellular RNA physiology we review evidence for naturally occurring anti sense RNA regulation. Although there are many examples of well charact erised overlapping RNA transcripts there is, as yet, no clear evidence suggesting complementary RNA-dependent gene regulation in S. cerevisi ae. The application of artificial antisense and ribozyme genes is then discussed with an emphasis on the role of yeast as a model system for the systematic and genetic analysis of antisense and ribozyme RNA fun ction. In addition, potential reasons for the lack of attempts to use antisense or ribozyme genes to create pseudogenetic mutants are consid ered. We conclude that the application of successful antisense and rib ozyme strategies in yeast may have to address features of S. cerevisia e RNA biology and offer experimental approaches that may identify some of these features.