GENES FOR DNA CYTOSINE METHYLTRANSFERASES AND STRUCTURAL PROTEINS, EXPRESSED DURING LYTIC GROWTH BY THE PHAGE PHI-ETA OF THE ARCHAEBACTERIUM HALOBACTERIUM-SALINARIUM
P. Stolt et al., GENES FOR DNA CYTOSINE METHYLTRANSFERASES AND STRUCTURAL PROTEINS, EXPRESSED DURING LYTIC GROWTH BY THE PHAGE PHI-ETA OF THE ARCHAEBACTERIUM HALOBACTERIUM-SALINARIUM, Biological chemistry Hoppe-Seyler, 375(11), 1994, pp. 747-757
Lytic genes and transcription from the Halobacterium salinarium phage
Phi H were studied. Genes for three structural proteins were located t
o the left arm of the linear phage genome. The right arm was shown to
encode three DNA cytosine methyltransferases, the first such sequences
reported from an archaebacterium. One cytosine methyltransferase is o
f the N(4)-methyltransferase type. The other two open reading frames (
ORFs) seem to be parts of the same gene, which has been split by a rec
ombination event. This gene product is of the C-5-methyltransferase ty
pe. The methyltransferase genes are the first Phi H genes detected sho
wing high homology to eubacterial proteins. Five of the six described
gene products have a higher proportion acidic over basic amino acid re
sidues, a common characteristic of halobacterial proteins. Lytic Phi H
transcription was shown to produce three RNA species, two shorter spe
cies encoding the methyltransferase genes and one large species transc
ribed from both the right and the left phage arm and subsequently bein
g processed upstream of the region encoding the structural proteins.