An updated human heart protein two-dimensional electrophoresis (2-DE)
database is presented. The database, which contains some 1388 protein
spots characterised in terms of M(r) and pI, has been analysed further
by Western immunoblotting and protein sequencing. From a total of 103
protein spots analysed, 49 have been identified by immunoblotting and
32 have been identified by protein sequencing. A further six proteins
have tentatively been assigned by comparison with the human heart 2-D
E protein database of Jungblut et al. (Electrophoresis) 1994, 15, 685-
607). This database is being used in studies of alterations in protein
expression in the diseased and transplanted human heart.