Lc. Duarte et al., DETERMINATION OF THE KINETIC-PARAMETERS IN CONTINUOUS CULTIVATION BY DEBARYOMYCES-HANSENII GROWN ON D-XYLOSE, Biotechnology techniques, 8(12), 1994, pp. 859-864
The kinetic parameters of the yeast Debaryomyces hansenii grown in con
tinuous cultivation on D-xylose were determined by different methods.
While the values obtained for mu(m) by the steady state and the washou
t methods only gave a 3% difference, the determined K-s values by the
steady state and the maximal biomass output methods led a. to a 305% d
ifference. The latter method was suggested to overestimate the K-s val
ue.