CHEMICAL STERILIZATION OF NUTRIENT MEDIA FOR PLANT-CELL CULTURES USING DIETHYLPYROCARBONATE

Authors
MACEK T KRAL J VANEK T BLAZEK J MACKOVA M
Citation
T. Macek et al., CHEMICAL STERILIZATION OF NUTRIENT MEDIA FOR PLANT-CELL CULTURES USING DIETHYLPYROCARBONATE, Biotechnology techniques, 8(12), 1994, pp. 885-888
Citations number
5
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
8
Issue
12
Year of publication
1994
Pages
885 - 888
Database
ISI
SICI code
0951-208X(1994)8:12<885:CSONMF>2.0.ZU;2-1
Abstract
Diethylpyrocarbonate (DPC), when added at about 1 g/l to culture media is able to sterilize them. DPC kills all the contaminating microorgan isms, and in contact with water it decomposes to ethanol and carbon di oxide in amounts not inhibiting the growth of plant cells. The plant c ells cultivated on media treated by DPC did not show changes in their basic characteristics.