STEM HEMATOPOIETIC-CELLS WITH INSERTED FOREIGN GENE - PROLIFERATIVE ACTIVITY AND PROLIFERATIVE POTENTIAL IN THE LONG-TERM AFTER TRANSPLANTATION INTO IRRADIATED MICE

The transfer of the human adenosine deaminase gene to murine stem hema topoietic cells is performed on an irradiated sublayer of a long-term bone marrow culture by the conventional method of retroviral transduct ion with cytokines and by stimulation of stem cells without cytokines. The efficiency of gene transfer into colony-forming units (CFUs) with the aid of cytokines is 72% and without them it is 50%. In irradiated mice reconstituted with the retrovirus-infected bone marrow cells the donor hematopoietic activity is preserved during a 1-year period. The proliferative activity of CFUs of chimeric cells 6 months after the r econstitution was the same and did not depend on the mode of gene tran sfer. The spleen repopulation activity is lowered in all the groups of chimeric mice 6-12 months after reconstitution.