DETERMINATION OF THE ABSOLUTE-CONFIGURATION OF (-NEOPENTYL-1-D ALCOHOL BY NEUTRON AND X-RAY-DIFFRACTION ANALYSIS())

The absolute configuration of (+)-neopentyl-1-d alcohol, prepared by t he reduction of 2,2-dimethylpropanal-1-d by actively fermenting yeast, has been determined to be S by neutron diffraction. The neutron study was carried out on the phthalate half ester of neopentyl-1-d alcohol, crystallized as its strychnine salt. The absolute configuration of th e (-)-strychninium cation was first determined by an x-ray anomalous d ispersion study of its iodide salt. The chiral skeleton of strychnine then served as a reference from which the absolute configuration of th e -O-CHD-C(CH3)(3) group of neopentyl phthalate was determined. Differ ence Fourier maps calculated from the neutron data showed unambiguousl y that the -O-CHD-C(CH3)(3) groups of both independent molecules in th e unit cell had the S configuration. This work proves conclusively tha t the yeast system reduces aldehydes by delivering hydrogen to the re face of the carbonyl group. Crystallographic details: (-)-strychninium (+)-neopentyl-1-d phthalate, space group P2(1) (monoclinic), a = 18.5 64(6) Angstrom, b = 7.713(2) Angstrom, c = 23.361(8) Angstrom, beta = 94.18(4)degrees, V = 3336.0(5) Angstrom(3), Z = 2 (T = 100 K). Final a greement factors are R(F) = 0.073 for 2768 reflections collected at ro om temperature (x-ray analysis) and R(F) = 0.144 for 960 reflections c ollected at 100 R (neutron analysis).