BORDETELLA HOLMESII SP-NOV, A NEW GRAM-NEGATIVE SPECIES ASSOCIATED WITH SEPTICEMIA

CDC nonoxidizer group 2 (NO-2) currently consists of 15 gram-negative, rod-shaped, oxidase-negative, asaccharolytic, brown soluble pigment-p roducing strains isolated from blood cultures, usually from young adul ts, On the basis of their cellular fatty acid profiles, NO 2 strains f ormed a single group that was identical with the profile of Bordetella avium. 16S rRNA sequencing of one NO-2 strain and the type strains of B. pertussis, B. parapertussis, B. bronchiseptica, and B. avium showe d a high degree of homology (greater than or equal to 98% over 1,525 b ases). The NO-2 guanine-plus-cytosine content (61.5 to 62.3 mol%) and major ubiquinone analysis (ubiquinone-8) results were both consistent with those for the genus Bordetella. DNA relatedness studies (hydroxya patite method) confirmed a close relatedness between NO-2 and Bordetel la species and demonstrated that NO-2 strains were a single ne rv spec ies. The name B. holmesii sp. nov. is proposed for CDC group NO-2.