PCR amplification of the 531-bp fragment of the Mycobacterium leprae p
ra gene in fresh biopsy and slit skin smear samples was evaluated for
its usefulness in the detection of leprosy bacilli in patients in Thai
land. In multibacillary patients, 87.1% (27 of 31) of biopsy specimens
and 41.9% (13 of 31) of slit skin smear specimens were positive by PC
R, whereas in paucibacillary patients, 36.4% (8 of 22) of biopsy speci
mens and 18.2% (4 of 22) of slit skin smear specimens yielded detectab
le PCR amplification. Compared with other diagnostic procedures, PCR s
howed a clear advantage over both microscopic examination of slit skin
smears and serologic detection of anti-phenolic glycolipid 1 antibody
, especially in paucibacillary patients when bacterial indexes were 0
and seropositivity was only 6.25%, PCR was also evaluated for its pote
ntial to help monitor bacterial clearance in some of these patients du
ring chemotherapeutic treatment, The PCR results on slit skin smear sa
mples at 1, 3, and 6 months of chemotherapy showed that the number of
PCR-positive cases of bath multibacillary and paucibacillary types dec
reased sequentially, The results of this study are encouraging, Howeve
r, investigation of a larger number of clinical specimens with an impr
ovement in PCR methods, especially on slit skin smears, needs to be do
ne before PCR can be established as a diagnostic procedure for leprosy
patients and subclinical cases or as a tool for drug assessment.