LOW HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1) DNA BURDEN AS A MAJORCAUSE FOR FAILURE TO DETECT HIV-1 DNA IN CLINICAL SPECIMENS BY PCR

Authors
ZAZZI M ROMANO L CATUCCI M DEMILITO A ALMI P GONNELLI A RUBINO M VALENSIN PE
Citation
M. Zazzi et al., LOW HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 (HIV-1) DNA BURDEN AS A MAJORCAUSE FOR FAILURE TO DETECT HIV-1 DNA IN CLINICAL SPECIMENS BY PCR, Journal of clinical microbiology, 33(1), 1995, pp. 205-208
Citations number
45
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
33
Issue
1
Year of publication
1995
Pages
205 - 208
Database
ISI
SICI code
0095-1137(1995)33:1<205:LHT(DB>2.0.ZU;2-R
Abstract
To determine the sensitivity of a nested PCR procedure for detecting h uman immunodeficiency virus type 1 DNA in clinical specimens, 553 peri pheral blood mononuclear cell samples obtained from 268 human immunode ficiency virus type 1-seropositive subjects were assayed by use of two independent primer sets for each sample. Overall, 1,088 of 1,106 (98. 37%) reactions were positive. Investigation of the negative reactions showed that a low viral burden in some infected subjects, rather than primer-template mismatches, was the primary cause for the false-negati ve PCR results.