MOLECULAR-GENETIC PROPERTIES OF THE YEAST TORULASPORA PRETORIENSIS - CHARACTERIZATION OF CHROMOSOMAL DNA AND GENETIC-TRANSFORMATION BY SACCHAROMYCES-CEREVISIAE-BASED PLASMIDS

Chromosomal DNA banding patterns were obtained for three strains of To rulaspora pretoriensis by contour-clamped homogenous-electric-field ge l electrophoresis. Chromosomes were resolved into six or seven bands i n the range of 800 to 2000 kb, and a polymorphism of these lengths was observed. By Southern-blot analysis, the three strains were shown to lack the DNA sequences homologous to the URA3, LEU2, TRP1, and HO gene s of Saccharomyces cerevisiae. A uracil auxotrophic mutant derived fro m T. pretoriensis was transformed with three plasmids (YEp24, YRpHI, a nd YCp50) carrying the URA3 gene of S. cerevisiae by the lithium aceta te method.