GLUCOSE-OXIDASE BIOSYNTHESIS USING MYCELIUM OF ASPERGILLUS-NIGER IMMOBILIZED ON SEEDS

Fungal conidia were immobilized on seeds of wheat, rye, barley, peas, buckwheat and mustards. Spongy mycelia overgrowing the seed surfaces o n mineral medium with glucose produced extracellular glucose oxidase ( GOD); the highest production was reached on the wheat carrier (1.3 U/m l). Some culture conditions, temperature, agitation speed, the amount of support were optimized to improve the growth and enzyme biosynthesi s by the immobilized mycelium. This procedure made also possible repea ted batch enzyme production. As many as 8 subsequent 24 h batches coul d be fermented by using the same carrier with only small losses of GOD activity. The new optimized medium with sodium nitrate caused a signi ficant increase in GOD activity produced by free cells of A. niger (19 3.6%) and by 122.4% in the case of the immobilized mycelium.