PROPENTOFYLLINE ENHANCEMENT OF THE ACTIONS OF ADENOSINE ON NEUTROPHILLEUKOCYTES

In agreement with previous results, activation of adenosine A, recepto rs was found to inhibit the exocytotic release of elastase and the oxi dative burst induced by formyl-MetLeuPhe (fMLP) in human neutrophils. The adenosine analogue 5'-N-ethylcarboxamidoadenosine (NECA) was more potent than adenosine (IC50 14 VS 64 nM). The effects of adenosine and NECA were not influenced by the A(1)-adenosine receptor selective ant agonist 1,3-dipropyl-8-cyclopentylxanthine (DFCPX; 300 nM), but were a bolished by the non-selective adenosine receptor antagonist ,6-dihydro -[1,2,4]-triazolo[1,5]quinazolin-5-imine monomethanesulfonate (CGS 159 43; 10 mu M). Propentofylline per se caused a concentration-dependent inhibition of H2O2 production. At 100 mu M propentofylline significant ly enhanced the effect of adenosine, but not that of NECA. This effect oi propentofylline was shared by the known uptake inhibitor dipyridam ole. Neither adenosine nor propentofylline altered fMLP-induced inosit ol-(1,4,5)-trisphosphate (IP3) formation. The results demonstrate that propentofylline can counteract neutrophil activation, at least partly by enhancing the action of adenosine through blocking its removal, an d that the effect is exerted at a step after the initial receptor even ts.