THYMIDYLATE SYNTHASE BINDS TO C-MYC RNA IN HUMAN COLON-CANCER CELLS AND IN-VITRO

Using an immunoprecipitation-reverse transcription-PCR technique, we c haracterized a thymidylate synthase (TS) ribonucleoprotein complex in cultured human colon cancer cells that consists of TS protein and the mRNA of the nuclear oncogene c-myc. TS protein is complexed in intact cells with the C-terminal coding region of c-myc mRNA that includes nu cleotide positions 1625 to 1790. RNA electrophoretic gel mobility shif t assays confirm a specific interaction between TS protein and c-myc m RNA and provide additional evidence that the C-terminal coding region represents an important cis-acting regulatory element. Further evidenc e demonstrates that the in vitro translational efficiency of c-myc mRN A is inhibited as a result of its direct interaction with TS protein. In addition, the presence of exogenous c-myc mRNA specifically relieve s the inhibitory effects of TS protein on TS mRNA translation.