USE OF ZWITTERIONIC BUFFER ADDITIVES TO IMPROVE THE SEPARATION OF PROTEINS IN CAPILLARY ZONE ELECTROPHORESIS

In CZE, the adsorption of the proteins on the capillary wall is a comm on problem. This paper describes the simple method of utilizing zwitte rionic buffer additives to Improve the separation of proteins in untre ated fused silica capillaries at neutral pH. Three kinds of zwitterion are evaluated in the separation of acidic, neutral, and basic protein s, including their effect on protein efficiency, mobility, separation, and resolution; the difference between the effects of the different a dditives are also highlighted. The method has proved to be a possible means of reducing protein adsorption, especially for basic proteins.