EXPRESSION OF THE ENZYMES OF NITRATE REDUCTION DURING THE ANAEROBIC GERMINATION OF RICE

Citation
M. Mattana et al., EXPRESSION OF THE ENZYMES OF NITRATE REDUCTION DURING THE ANAEROBIC GERMINATION OF RICE, Plant physiology, 106(4), 1994, pp. 1605-1608
Citations number
26
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
00320889
Volume
106
Issue
4
Year of publication
1994
Pages
1605 - 1608
Database
ISI
SICI code
0032-0889(1994)106:4<1605:EOTEON>2.0.ZU;2-Z
Abstract
During the anaerobic germination of rice (Oryza sativa L.), nitrate is translocated from the caryopsis and assimilated into the coleoptile ( R. Reggiani, M. Mattana, N. Aurisano, A. Bertani [1993] Plant Cell Phy siol 34: 379-383). Using antibodies against nitrate and nitrite reduct ases, proteins with the expected molecular mass were recognized by wes tern blot analysis in extracts from 8-d-old rice coleoptiles. Both enz ymes are de novo synthesized in 6- to 8-d-old seedlings, as shown by i mmunoprecipitation of radiolabeled proteins from young plants grown in the presence of [S-35]methionine. The anaerobic synthesis of both enz ymes was enhanced by the addition of 5 mM KNO3. The effect of exogenou s nitrate on the expression of the corresponding genes in anaerobic ri ce coleoptiles was revealed by the analysis of their transcripts. The importance of the expression of these enzymes during the anaerobic dev elopment of rice seedlings is discussed.