J. Kimura et al., LOCALIZATION OF ZP3 MESSENGER-RNA IN MOUSE OVARY BY NONRADIOACTIVE IN-SITU HYBRIDIZATION WITH DIGOXIGENIN-LABELED CDNA, Cellular and molecular biology, 40(8), 1994, pp. 1097-1101
In situ hybridization using digoxigenin-labelled cDNA has dual advanta
ges of having high structural resolution and high sensitivity and avoi
ding problems associated with the handling of radioactive materials. W
e have used this technique to detect and locate mRNA for mouse ZP3 pro
tein in paraffin sections of fixed ovaries of newborn and adult mice.
Mouse ZP3 mRNA was restricted to the cytoplasm of oocytes. After day 1
6, ZP3 mRNA expression was increasingly restricted to the cytoplasm of
the oocyte. Previous work on the distribution of ZP3 mRNA was confirm
ed and we were able to show that using non-isotopic in situ hybridizat
ion, sensitive and accurate cellular localization was feasible in para
ffin embedded mouse ovary.