IDENTIFICATION OF DISULFIDE BRIDGES IN A CARDIOTOXIC PEPTIDE BY ELECTROSPRAY-IONIZATION

Authors
KRISHNAMURTHY T HAUER CR PRABHAKARAN M FREEDY JG HAYASHI K
Citation
T. Krishnamurthy et al., IDENTIFICATION OF DISULFIDE BRIDGES IN A CARDIOTOXIC PEPTIDE BY ELECTROSPRAY-IONIZATION, Biological mass spectrometry, 23(12), 1994, pp. 719-726
Citations number
19
Categorie Soggetti
Spectroscopy,Biophysics
Journal title
Biological mass spectrometry → ACNP
ISSN journal
10529306
Volume
23
Issue
12
Year of publication
1994
Pages
719 - 726
Database
ISI
SICI code
1052-9306(1994)23:12<719:IODBIA>2.0.ZU;2-6
Abstract
A new method was developed for subjecting a peptide to a specified num ber of Edman degradation cycles on an automated polypeptide sequencer and desorbing the residual peptide for further investigations, The pro cedure was applied in combination with electrospray ionization mass sp ectrometry to identify the four disulfide bridges present in a small t ightly bound peptide, The task was accomplished using only a few nanom oles of the intact peptide,