STRUCTURAL CHARACTERIZATION OF SYNTHETIC MODEL PEPTIDES OF THE DNA-BINDING CI434 REPRESSOR BY ELECTROSPRAY-IONIZATION AND FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY

Authors
PERCIPALLE P SALETTI R PONGOR S FOTI S TOSSI A FISICHELLA S
Citation
P. Percipalle et al., STRUCTURAL CHARACTERIZATION OF SYNTHETIC MODEL PEPTIDES OF THE DNA-BINDING CI434 REPRESSOR BY ELECTROSPRAY-IONIZATION AND FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY, Biological mass spectrometry, 23(12), 1994, pp. 727-733
Citations number
11
Categorie Soggetti
Spectroscopy,Biophysics
Journal title
Biological mass spectrometry → ACNP
ISSN journal
10529306
Volume
23
Issue
12
Year of publication
1994
Pages
727 - 733
Database
ISI
SICI code
1052-9306(1994)23:12<727:SCOSMP>2.0.ZU;2-V
Abstract
The structural characterization of two synthetic model peptides of the cI434 repressor is described. Unequivocal determination of the struct ure was achieved by means of electrospray ionization mass spectrometry of the intact peptides and by fast atom bombardment mass spectrometri c identification of complementary peptide fragments obtained by trypti c and chymotrypic digestion and partial separation by reversed-phase h igh-performance liquid chromatography. The results show the potential of this approach for characterizing synthetic peptides of relatively h igh molecular weight.