PEPTIDE SEQUENCE INFORMATION DERIVED BY PARTIAL ACID-HYDROLYSIS AND MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS-SPECTROMETRY/

Partial acid hydrolysis of purified peptides followed by matrix-assist ed laser desorption/ionization time-of-flight mass spectrometry of the resulting peptide mixture is a fast and simple procedure to confirm t he identity of a peptide tentatively identified by its determined mole cular weight. Often partial amino acid sequence information can be obt ained directly. The sensitivity of the technique is in the high-femtom ole to low-picomole range.