LARGE-SCALE PRODUCTION AND SEMI-PURIFICATION OF KEDARCIDIN IN A 1000-L FERMENTER

Actinomycete strain ATCC 53650 was grown in a 1000-L fermentor contain ing 680 L of medium and the production of kedarcidin was monitored by HPLC. The titers of kedarcidin in the fermentor cultures were 0.49-0.5 3 mg ml-1. A quick and efficient purification method involving the use of anion exchange resin DE23 (batch adsorption-desorption) and ultraf iltration system yielded high recovery (65% yield) of kedarcidin from the fermentor culture. Over 200 grams of lyophilized kedarcidin of 70% purity was recovered from each of two 1000-L fermentor cultures using this process.