J. Lehrfeld, HPLC SEPARATION AND QUANTITATION OF PHYTIC ACID AND SOME INOSITOL PHOSPHATES IN FOODS - PROBLEMS AND SOLUTIONS, Journal of agricultural and food chemistry, 42(12), 1994, pp. 2726-2731
A macroporous polymer column was used to resolve a mixture of inositol
hexakis-, pentakis-, tetrakis-, and trisphosphates within 5-9 min. Op
timized eluting systems based on acetonitrile or methanol had improved
resolution and reduced chromatographic run times. The silica-based an
ion exchange column used in the analysis of cereal samples was used to
prepare small amounts of pure inositol pentakis-, tetrakis-, and tris
phosphates for calibration standards. A number of potential analytical
problems are discussed, and solutions are proposed.