N. Aharonson et al., SUPERCRITICAL-FLUID EXTRACTION AND HPLC ANALYSIS OF BENZIMIDAZOLE FUNGICIDES IN POTATO, APPLE, AND BANANA, Journal of agricultural and food chemistry, 42(12), 1994, pp. 2817-2823
An analytical method employing supercritical fluid extraction (SFE) of
the benzimidazole fungicides thiabendazole, benomyl/carbendazim, and
thiophanate-methyl was developed and tested with potato, banana, and a
pple matrices. Ion-pairing HPLC coupled with UV absorbance or fluoresc
ence detection was used for analysis. Sample preparation involved shre
dding a 100 g sample, mixing a 3 g subsample with 2 g of Hydromatrix,
and packing a 10 mL vessel. Optimal SFE conditions were determined by
varying the amount, density, and flow rate of CO2, extraction temperat
ure, type and amount of solvent modifier, and trapping/elution conditi
ons of the extracted pesticides. Optimal conditions for extraction wer
e obtained at 400 atm, 60 degrees C, 90 g of CO2, 3% methanol modifier
, and 2 mL/min flow rate. The extracts were collected on a 1 mL C-18 t
rap at 25 degrees C and eluted with 10 mL of methanol. Potato, apple,
and banana extracts were analyzed by HPLC without additional cleanup.
Recoveries were 90 +/- 12% for the benzimidazole fungicides in fortifi
ed samples. Incurred potato and banana samples were also analyzed.