TRANSFORMATION OF BRUSSELS-SPROUTS (BRASSICA-OLERACEA VAR GEMMIFERA ZENK) BY AGROBACTERIUM-RHIZOGENES HARBORING A REPORTER, BETA-GLUCURONIDASE GENE

Transfer of beta-glucuronidase (GUS) gene into Brussels sprouts was ma de using Agrobacterium rhizogenes vector-system. Plasmid pBI 121 was t ransferred to A. rhizogenes IFO 13257 by the freeze-thaw method. When leaves of Brussels sprouts were inoculated with Agrobacterium, 34 hair y roows were produced with GUS activities. From seven root clones with GUS activities, 14 shoots were regenerated from calli on Murashige-Sk oog (MS) medium supplemented with 1 mg.liter(-1) alpha-naphthaleneacet ic acid (NAA) plus 5 mg.liter(-1) zeatin. Seven shoots obtained from f our root clones again showed GUS activities, suggesting that they were transformants. In two root clones, shoots with and without GUS activi ty were regenerated from the same callus. Four shoots from three root clones showed no activity. Thus, half of GUS genes were stably transmi tted from hairy roots to regenerated plants.