A method was developed to determine aflatoxin M(1) in Parmesan cheese
produced in the Modena area during 1991. The analytical method was bas
ed on extraction of aflatoxin M(1) from the cheese by chloroform and c
lean-up by liquid-liquid extraction. The determination was made by hig
h-pressure liquid chromatography. Of 200 fresh Parmesan cheese samples
analyzed, 18 showed presence of aflatoxin M(1) in low concentrations
(max. 0.190 mu g/kg). Italian food law does not establish any maximum
allowed level for this aflatoxin. The maximum limit proposed by the Sw
iss Federal Bureau of Health was 0.250 mu g/kg.