ENANTIOMER SEPARATION BY THE CAPILLARY EL ECTROKINETIC TECHNIQUE

As with chromatographic methods, capillary electrokinetic techniques s uch as zone electrophoresis, gel electrophoresis, micellar electrokine tic chromatography and electrochromatography have been shown to be suc cessful in chiral separations. These techniques rely on either pre-inj ection diastereoisomer formation (indirect mode) or on formation of di astereoisomer during the separation (direct mode). Thus far, most enan tiomer separations have been done in the direct mode with the chiral a gent present in the electrolyte. Four chiral agent groups have been te sted: metal complexes with chiral ligands, inclusion agents (cyclodext rins, crown-ethers), chiral micelles, proteins and glycoproteins. The mechanism of chiral recognition for each group is presented and most o f the proven applications are discussed.