Jw. Breneman et al., THE DEVELOPMENT OF PAINTING PROBES FOR DUAL-COLOR AND MULTIPLE CHROMOSOME ANALYSIS IN THE MOUSE, Cytogenetics and cell genetics, 68(3-4), 1995, pp. 197-202
The recent development of mouse chromosome painting probes for fluores
cence in situ hybridization has extended the use of this common labora
tory mammal in cytogenetics. We now report the development of addition
al painting probes by degenerate-oligonucleotide-primed PCR on chromos
omes from mouse lung fibroblast cultures, each homozygous for a single
Robertsonian translocation chromosome. These probes are for Rb(1.2),
Rb(1.3), Rb(4.6), and Rb(6.7). Probes were also made for the sex chrom
osomes by isolating shoulders from larger peaks (X) or small, clearly
resolved peaks (Y) in the flow karyotype. Combinations of probes were
used to paint four chromosomes simultaneously in a single color. Multi
color painting was achieved with a biotinylated Rb(1.2) probe and a di
goxigenin-labeled Rb(2.8) probe. Each of the three different homologou
s pairs was uniquely colored by avidin-Texas Red, anti-digoxigenin-FIT
C, or both simultaneously. These results extend the usefulness of the
mouse as a model for understanding adverse environmental exposures and
genetic diseases in humans.