MATURATION OF NONADRENERGIC NONCHOLINERGIC INHIBITORY SYSTEM IN NORMAL AND ALLERGEN-SENSITIZED RABBITS

We investigated the functional existence of the nonadrenergic noncholi nergic inhibitory (NANCi) system in developing rabbit airways in vitro . Furthermore, we evaluated the effect of parenteral exposure to a spe cific allergen (ragweed) on the maturation of this neural pathway. NAN Ci responses were studied on tracheal smooth muscle (TSM) segments obt ained from normal and ragweed-sensitized New Zealand White rabbits at 1, 2, 4, and 12 wk of age. The TSM segments were removed and placed in tissue baths containing modified Krebs-Henseleit solution, atropine ( 1 x 10(-5) M), and propranolol (5 x 10(-6) M). After contraction with neurokinin A (1 x 10(-5) M), electrical field stimulation was applied at stimulation frequencies ranging from 5 to 30 Hz to determine the fr equency that produced maximal relaxation. The NANCi response to EFS wa s measured and expressed as the mean (+/-SE) percent relaxation at 20 Hz, because this stimulation frequency gave the maximal NANCi response at each age studied. TSM segments obtained from control rabbits at 1 wk of age did not demonstrate a NANCi response at the frequencies of s timulation used. By contrast, a reproducible NANC relaxation was demon strated in TSM from 2-, 4-, and 12-wk-old rabbits. The magnitude of th is response was 27 +/- 4.7 (n = 10), 29 +/- 4.8 (n = 9), and 37 +/- 4% (n = 18), respectively. The same experiments performed on TSM segment s obtained from ragweed-sensitized animals gave significantly decrease d values of NANCi response. In 2-, 4-, and 12-wk-old rabbits, the NANC i responses were 11.5 +/- 3.4 (n = 9), 11 +/- 2 (n = 13), and 16 +/- 4 .2% (n = 14). Again, no NANC relaxation was present at 1 wk of age. Th ese findings suggest that NANCi innervation is not functional at birth and undergoes considerable postnatal development. Furthermore, the ma turation of the NANCi system is impaired in ragweed-sensitized rabbits . This early dysfunction may disrupt the airways' neural control and c ontribute to the development of altered airways function.