PERIPHERAL-BLOOD PROGENITOR-CELL TRANSPLANTATION ESTIMATED BY 3-COLOR(CD34, HLA-DR, CD33) FLOW-CYTOMETRY

The relationships between transfused cell number of CD34+ cell subpopu lations divided by HLA-DR and CD33 antibodies and hematopoietic recove ry patterns after peripheral blood progenitor cell transplantation (PB PCT) subsequent to myeloablative chemoradiotherapy were investigated i n 14 children with cancer. Both logarithm of transfused CD34+ cell num ber/10(6)/kg and logarithm of transfused cell number/10(6)/kg of the C D34+HLA-DR+CD33+ subpopulation, which is supposed to be myeloid-commit ted cells, were correlated with myeloid recovery after PBSCT, though t hey were not correlated with erythroid or platelets recovery. On the o ther hand, logarithm of transfused cell number/10(6)/kg of CD34+HLA-DR -CD33-subpopulation, which is supposed to be immature progenitor cells , was not correlated with myeloid recovery but correlated with erythro id recovery and platelet recovery. These results suggested that rapid myelopoiesis after PBPCT occurs following transfusion of sufficient nu mbers of myeloid-committed cells and complete hematopoietic reconstitu tion occurs after transfusion of sufficient numbers of immature hemato poietic progenitor cells.