H. Rabb et al., ROLE OF CD11A AND CD11B IN ISCHEMIC ACUTE-RENAL-FAILURE IN RATS, American journal of physiology. Renal, fluid and electrolyte physiology, 36(6), 1994, pp. 60001052-60001058
Leukocytes, particularly neutrophils, have been implicated in ischemic
-reperfusion organ injury (IRI). However,. their role in kidney IRI is
controversial. Leukocytes express the adhesion molecules CD11/CD18 on
their surface, which mediate many functions that can lead to tissue d
amage. To determine the role of CD11a and CD11b in IRI in the kidney,
uninephrectomized Sprague-Dawley rats were pretreated with monoclonal
antibodies (MAbs) directed against CD11a and CD11b or control MAbs. Th
e serum creatinine (SCr), complete blood count, and kidney histopathol
ogical damage scores (PDS) (scale: 0-4) were assessed prior to and 24
h after 60 min of ischemia. Mean SCr 24 h after ischemia was significa
ntly decreased in the anti-CD11a- and -CD11b-treated group compared wi
th the control MAb-treated group (2.5 +/- 0.3 mg/dl vs. 3.4 +/- 0.2 mg
/dl, P < 0.05). PDS were also reduced in the CD11a and CD11b group com
pared with controls (2.7 +/- 0.2 vs. 3.5 +/- 0.1, P < 0.001). These da
ta show that the CD11/CD18 leukocyte adhesion pathway plays a role in
mediating ischemic acute renal failure in rats.