Altogether 11 geographic populations of Drosophila kikkawai were analy
sed from the viewpoint of karyotypic variation, inversion polymorphism
and sexual isolation. The results revealed that of all the population
s, the New Guinea population was the most genetically diverged, charac
terized by its distinct karyotype, possession of a fixed inversion, an
d its females exhibiting sexual isolation at the level of preferential
mating. Based on these findings it was inferred that incipient specia
tion has proceeded in D. kikkawai and its New Guinea population is on
its way to speciation.