INTERACTION OF RNASE-H FROM ESCHERICHIA-C OLI WITH MODIFIED OLIGONUCLEOTIDE HYBRID DUPLEXES .1. DUPLEXES CONTAINING MODIFICATION IN FURANOSE MOIETY

Authors
KRYNETSKAYA NF ALEKSEEV YI BELKOV VM IBRAHIM HCH TASHLITSKY VN ORETSKAYA TS SHABAROVA ZA
Citation
Nf. Krynetskaya et al., INTERACTION OF RNASE-H FROM ESCHERICHIA-C OLI WITH MODIFIED OLIGONUCLEOTIDE HYBRID DUPLEXES .1. DUPLEXES CONTAINING MODIFICATION IN FURANOSE MOIETY, Bioorganiceskaa himia, 20(11), 1994, pp. 1218-1225
Citations number
12
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
20
Issue
11
Year of publication
1994
Pages
1218 - 1225
Database
ISI
SICI code
0132-3423(1994)20:11<1218:IORFEO>2.0.ZU;2-2
Abstract
The influence of oligodeoxyribonucleotide probes containing 1-(D-beta- 2'-deoxy-treo-pentofuranosyl)thymine or 1-(D-beta-2'-deoxy-2'-fluoro-p entofuranosyl)uracil on the ability of the hybrid duplexes to interact with RNase H from E. coli was studied. A kinetic approach was used to measure of the modification effect. The hybrid duplex, prA18/d(TTflU) 6TT, was shown not to interact with RNase H, whereas prA18/d(xTTT)6 in hibited the RNase H activity (K(i) = 0.67 mkM). The thermostability of the modified duplexes was estimated. The present technique may lead t o the use of some modified oligonucleotides as antisences.