INTRACELLULAR LOCATION OF THE EPITOPES OF 2 NEW MONOCLONAL-ANTIBODIESAGAINST THE VAL(928)-LYS(945) FRAGMENT OF THE ALPHA-SUBUNIT OF NA-ATPASE - AN APPLICATION TO TOPOLOGICAL MODELS(,K+)

Authors
LARIN DI SHAKHPARONOV MI ORTIZ E KOSTINA MB MODYANOV NN
Citation
Di. Larin et al., INTRACELLULAR LOCATION OF THE EPITOPES OF 2 NEW MONOCLONAL-ANTIBODIESAGAINST THE VAL(928)-LYS(945) FRAGMENT OF THE ALPHA-SUBUNIT OF NA-ATPASE - AN APPLICATION TO TOPOLOGICAL MODELS(,K+), Biologiceskie membrany, 11(6), 1994, pp. 605-613
Citations number
33
Categorie Soggetti
Cell Biology
Journal title
Biologiceskie membrany → ACNP
ISSN journal
02334755
Volume
11
Issue
6
Year of publication
1994
Pages
605 - 613
Database
ISI
SICI code
0233-4755(1994)11:6<605:ILOTEO>2.0.ZU;2-U
Abstract
Two monoclonal antibodies against the Val928-Lys945 fragment of the al pha-subunit of pig Na+,K+-ATPase were obtained. The location of their epitopes was determined inside the cell by the enzyme-linked immunoass ay method using intact and inverted cells (erythrocytes and pig embryo kidney cells) as antigens. By means of chemical modification and enzy matic degradation of the Val928-Lys945 fragment, the epitopes of these monoclonal antibodies were determined in detail. Based on the obtaine d data, models of the transmembrane organization of the COOH-terminal region of Na+,K+-ATPase alpha-subunit were proposed.